The Edward-Wakool river system is a major anabranch of the Murray River and has been intensively studied since 2010, including as a study site for the Long-Term Intervention Monitoring program. This site was impacted by a major bloom of the cyanobacteria Chrysosporum ovalisporum during February-June 2016. Water quality parameters including dissolved oxygen, temperature, pH, nutrients, dissolved organic carbon, carbon spectroscopic properties, chlorophyll-a and algal biovolumes were measured weekly and compared to baseline data from previous seasons. Water quality was strongly impacted by the bloom, including increases in total nitrogen and total phosphorus following the onset of the bloom and intermittent rises in NOx and ammonia. Changes in dissolved organic carbon were not well correlated to the absorbance or fluorescence of the water samples and are not reliable predictors of bloom collapse.